Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells.

ABSTRACT: Pluripotent stem cells (PSCs) have various degrees of pluripotency, which necessitates selection of PSCs with high pluripotency before their application to regenerative medicine. However, the quality control processes for PSCs are costly and time-consuming, and it is essential to develop inexpensive and less laborious selection methods for translation of PSCs into clinical applications. Here we developed an imaging system, termed Phase Distribution (PD) imaging system, which visualizes subcellular structures quantitatively in unstained and unlabeled cells. The PD image and its derived PD index reflected the mitochondrial content, enabling quantitative evaluation of the degrees of somatic cell reprogramming and PSC differentiation. Moreover, the PD index allowed unbiased grouping of PSC col

SUBMITTER: Nishimura K 

PROVIDER: S-EPMC6370783 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature