Automated microfluidic cell culture of stem cell derived dopaminergic neurons.

ABSTRACT: Parkinson's disease is a slowly progressive neurodegenerative disease characterised by dysfunction and death of selectively vulnerable midbrain dopaminergic neurons and the development of human in vitro cellular models of the disease is a major challenge in Parkinson's disease research. We constructed an automated cell culture platform optimised for long-term maintenance and monitoring of different cells in three dimensional microfluidic cell culture devices. The system can be flexibly adapted to various experimental protocols and features time-lapse imaging microscopy for quality control and electrophysiology monitoring to assess cellular activity. Using this system, we continuously monitored the differentiation of Parkinson's disease patient derived human neuroepithelial stem cells into

SUBMITTER: Kane KIW 

PROVIDER: S-EPMC6370836 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature