Cytotoxicity of apo bovine α-lactalbumin complexed with La³⁺ on cancer cells supported by its high resolution crystal structure.

ABSTRACT: Cancer remains one of the biggest threats to human society. There are massive demands for compounds to selectively kill cancerous cells. Earlier studies have shown that bovine α -lactalbumin made lethal to tumor cells (BAMLET) becomes cytotoxic against cancer cells in complex with oleic acid {Hoque, M. et. al., PLoSOne 8, e68390 (2013)}. In our study, we obtained bovine α-lactalbumin complexed with lanthanum ion (La³⁺-B-α-LA) and determined its high resolution crystal structure. The natural calcium binding site of bovine α-lactalbumin is replaced by lanthanum. The La³⁺ complex formation by B-α-apo-LA was also supported by various biophysical methods. Interestingly, our complex, La³⁺-B-α-LA exhibits much greater anticancer activity against breast cancer cell

SUBMITTER: Yarramala DS 

PROVIDER: S-EPMC6370903 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature