Epigenetic Status of The Human MMP11 Gene Promoter is Altered in Patellar Tendinopathy.

ABSTRACT: Patellar tendinopathy (PT) is a debilitating condition that often affects those who are physically active. Gene variation is known to contribute to human tendinopathy but the role of DNA methylation, as an epigenetic factor, has only recently been discovered. Using a case-control approach, we sought to determine whether differences existed between the methylation status of the MMP11 gene promoter in patellar tendinopathy compared to healthy tendon. We used PCR and pyrosequencing to interrogate the methylation profiles of 4 CpG sites (areas of the genome rich in C/G nucleotides) upstream of the MMP11 gene in DNA from males with PT (n = 10) and those with healthy tendon (n = 10). We also conducted a correlation analysis to establish whether age influenced methylation in the PT

SUBMITTER: Rickaby R 

PROVIDER: S-EPMC6370951 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature