Imperfect repeats in the functional amyloid protein FapC reduce the tendency to fragment during fibrillation.

ABSTRACT: Functional amyloid (FA) is widespread in bacteria and serves multiple purposes such as strengthening of biofilm and contact with eukaryotic hosts. Unlike pathological amyloid, FA has been subjected to evolutionary optimization which is likely to be reflected in the aggregation mechanism. FA from different bacteria, including Escherichia coli (CsgA) and Pseudomonas (FapC), contains a number of imperfect repeats which may be key to efficient aggregation. Here we report on the aggregative behavior of FapC constructs which represent all single, double, and triple deletions of the protein's three imperfect repeats. Analysis of the fibrillation kinetics by the program Amylofit reveals that the removal of these repeats increases the tendency of the growing fibrils to fragment and also generally i

SUBMITTER: Rasmussen CB 

PROVIDER: S-EPMC6371220 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature