Cancer-cell-secreted CXCL11 promoted CD8⁺ T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC.

ABSTRACT:

Background

Chemotherapy combined with immunotherapy becomes the main trend in lung cancer intervention; however, how chemotherapy promotes the immune function remains elusive. Therefore, we sought to determine how chemotherapy promotes the immune function.

Methods

We determined in 100 NSCLC patients the expression of CD8, functional markers (IFN-γ, Granzyme B, and Perforin) and specific chemokines by quantitative real-time reverse transcriptase-PCR. Functional experiments were carried out to check whether docetaxel (DOC), a chemotherapeutic agent, modifies the expression of HMGB1 and CXCL11, and influences the infiltration properties of CD8⁺ T cells to the tumor microenvironment. The mechanism of the release of HMGB1 and CXCL11 was determined by flow cytometry, im

SUBMITTER: Gao Q 

PROVIDER: S-EPMC6371476 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature