Establishment and characterization of the GC-030-35 cell line derived from gastric hepatoid adenocarcinoma.

ABSTRACT:

Purpose

Gastric hepatoid adenocarcinoma is a rare subtype of primary gastric cancer and is a high-grade form of malignancy. However, the pathogenesis and molecular biology of gastric hepatoid adenocarcinoma remain poorly understood. The aim of this study was to establish and characterize a new human gastric hepatoid adenocarcinoma cell line, GC-030-35.

Materials and methods

The GC-030-35 cell line was established from tumor cells from a 58-year-old Chinese man with gastric hepatoid adenocarcinoma. The cultured cells underwent immunocytochemistry and flow cytometry to confirm the tumor cell phenotype. RNA sequencing was performed to analyze the differences in gene expression between GC-030-35 cells compared with normal gastric epithelial cells. A zebrafish assay was performed

SUBMITTER: Wei J 

PROVIDER: S-EPMC6371931 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature