Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection.

ABSTRACT: Simple, fast, and precise counting of viable bacteria is fundamental to a variety of microbiological applications such as food quality monitoring and clinical diagnosis. To this end, agar plating, microscopy, and emerging microfluidic devices for single bacteria detection have provided useful means for counting viable bacteria, but they also have their limitations ranging from complexity, time, and inaccuracy. We present herein our new method RAPiD (Resazurin-Amplified Picoarray Detection) for addressing this important problem. In RAPiD, we employ vacuum-assisted sample loading and oil-driven sample digitization to stochastically confine single bacteria in Picoarray, a microfluidic device with picoliter-sized isolation chambers (picochambers), in <30 s with only a few minutes of hands-on t

SUBMITTER: Hsieh K 

PROVIDER: S-EPMC6372093 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature