Local Tension on Talin in Focal Adhesions Correlates with F-Actin Alignment at the Nanometer Scale.

ABSTRACT: Cellular force transmission and mechanotransduction are critical in embryogenesis, normal physiology, and many diseases. Talin plays a key role in these processes by linking integrins to force-generating actomyosin. Using the previously characterized FRET-based talin tension sensor, we observed variations of tension both between and within individual focal adhesions in the same cell. Assembling and sliding adhesions showed gradients with higher talin tension toward the cell center, whereas mature, stable adhesions had uniform talin tension. Total talin accumulation was maximal in high-tension regions; by contrast, vinculin intensity was flat or maximal at the adhesion center, and actin intensity was maximal toward the cell center. To investigate mechanism, we combined talin tension imaging

SUBMITTER: Kumar A 

PROVIDER: S-EPMC6372196 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature