Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling.

ABSTRACT: Positive-sense RNA viruses hijack intracellular membranes that provide niches for viral RNA synthesis and a platform for interactions with host proteins. However, little is known about host factors at the interface between replicase complexes and the host cytoplasm. We engineered a biotin ligase into a coronaviral replication/transcription complex (RTC) and identified >500 host proteins constituting the RTC microenvironment. siRNA-silencing of each RTC-proximal host factor demonstrated importance of vesicular trafficking pathways, ubiquitin-dependent and autophagy-related processes, and translation initiation factors. Notably, detection of translation initiation factors at the RTC was instrumental to visualize and demonstrate active translation proximal to replication complexes of several

SUBMITTER: V'kovski P 

PROVIDER: S-EPMC6372286 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature