West Nile Virus Capsid Protein Interacts With Biologically Relevant Host Lipid Systems.

ABSTRACT: West Nile and dengue viruses are closely related flaviviruses, originating mosquito-borne viral infections for which there are no effective and specific treatments. Their capsid proteins sequence and structure are particularly similar, forming highly superimposable α-helical homodimers. Measuring protein-ligand interactions at the single-molecule level yields detailed information of biological and biomedical relevance. In this work, such an approach was successfully applied on the characterization of the West Nile virus capsid protein interaction with host lipid systems, namely intracellular lipid droplets (an essential step for dengue virus replication) and blood plasma lipoproteins. Dynamic light scattering measurements show that West Nile virus capsid protein binds very low-density lipo

SUBMITTER: Martins AS 

PROVIDER: S-EPMC6372508 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature