Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein.

ABSTRACT: Biological characterisation of membrane proteins lags behind that of soluble proteins. This reflects issues with the traditional use of detergents for extraction, as the surrounding lipids are generally lost, with adverse structural and functional consequences. In contrast, styrene maleic acid (SMA) copolymers offer a detergent-free method for biological membrane solubilisation to produce SMA-lipid particles (SMALPs) containing membrane proteins together with their surrounding lipid environment. We report the development of a reverse-phase LC-MS/MS method for bacterial phospholipids and the first comparison of the profiles of SMALP co-extracted phospholipids from three exemplar bacterial membrane proteins with different topographies: FtsA (associated membrane protein), ZipA (single transme

SUBMITTER: Teo ACK 

PROVIDER: S-EPMC6372662 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature