Flotillin1 promotes EMT of human small cell lung cancer via TGF-β signaling pathway.

ABSTRACT:

Objective

Small cell lung carcinoma (SCLC) is considered one of the most aggressive types of lung cancer due to its rapid growth and early metastasis. No tumor markers or therapeutic targets have been demonstrated to be specific or effective in SCLC to date. This study aims to evaluate the potential of Flotillin1 (Flot1) as a target of SCLC treatment.

Methods

Flot1 expression level in the tissue of SCLC and other tissue of lung disease was detected using immunohistochemical staining. Transwell and Matrigel assays were employed to examine migration and invasion of cancer cells. Flow cytometry and xCELLigence system were used to evaluate cell apoptosis and cell viability, respectively. Expression levels of Flot1, epithelial-mesenchymal transition (EMT) marker E-cadherin, vimen

SUBMITTER: Zhao L 

PROVIDER: S-EPMC6372910 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature