Quantitative Comparison of Enrichment from DNA-Encoded Chemical Library Selections.

ABSTRACT: DNA-encoded chemical libraries (DELs) provide a high-throughput and cost-effective route for screening billions of unique molecules for binding affinity for diverse protein targets. Identifying candidate compounds from these libraries involves affinity selection, DNA sequencing, and measuring enrichment in a sample pool of DNA barcodes. Successful detection of potent binders is affected by many factors, including selection parameters, chemical yields, library amplification, sequencing depth, sequencing errors, library sizes, and the chosen enrichment metric. To date, there has not been a clear consensus about how enrichment from DEL selections should be measured or reported. We propose a normalized  z-score enrichment metric using a binomial distribution model that satisfies important crit

SUBMITTER: Faver JC 

PROVIDER: S-EPMC6372980 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature