SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is the most major type of primary hepatic cancer. Serine hydroxymethyltransferase 1 (SHMT1) is recently found to play critical roles in human cancers including lung cancer, ovarian cancer and intestinal cancer. However, the expression, function and the underlying mechanisms of SHMT1 in HCC remain uncovered.

Methods

qRT-PCR, immunohistochemistry and immunoblotting were performed to detect the expression of SHMT1 in HCC tissues and cell lines. HCC cell migration and invasion were determined by Boyden chamber and Transwell assay in vitro, and tumor metastasis was assessed via lung metastasis model in mice. The expression of key factors involved in epithelial-to-mesenchymal transition (EMT) process was evaluated by western blotting.

R

SUBMITTER: Dou C 

PROVIDER: S-EPMC6373090 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature