Rapid molecular detection of macrolide resistance.

ABSTRACT:

Background

Emerging antimicrobial resistance is a significant threat to human health. However, methods for rapidly diagnosing antimicrobial resistance generally require multi-day culture-based assays. Macrolide efflux gene A, mef(A), provides resistance against erythromycin and azithromycin and is known to be laterally transferred among a wide range of bacterial species.

Methods

We use Recombinase Polymerase Assay (RPA) to detect the antimicrobial resistance gene mef(A) from raw lysates without nucleic acid purification. To validate these results we performed broth dilution assays to assess antimicrobial resistance to erythromycin and ampicillin (a negative control).

Results

We validate the detection of mef(A) in raw lysates of Streptococcus pyogenes, S. pneumoniae, S

SUBMITTER: Nelson MM 

PROVIDER: S-EPMC6373131 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature