PRIMA-1^MET-induced neuroblastoma cell death is modulated by p53 and mycn through glutathione level.

ABSTRACT:

Background

Neuroblastoma is the most common extracranial solid tumor in children. This cancer has a low frequency of TP53 mutations and its downstream pathway is usually intact. This study assessed the efficacy of the p53 activator, PRIMA-1^MET, in inducing neuroblastoma cell death.

Methods

CellTiter 2.0 was used to study susceptibility and specificity of NB cell lines to PRIMA-1^MET. Real-time PCR and western blot were used to assess the most common p53 transactivation targets. Induction of p53 and Noxa, and inhibition of Cas3/7, were used to assess impact on cell death after PRIMA-1^MET treatment. Flow cytometry was used to analyze cell cycle phase and induction of apoptosis, reactive oxygen species, and the collapse of mitochondrial membrane

SUBMITTER: Mlakar V 

PROVIDER: S-EPMC6373164 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature