Disruption of GPR35 Exacerbates Dextran Sulfate Sodium-Induced Colitis in Mice.

ABSTRACT:

Background

G protein-coupled receptor 35 (GPR35) is an orphan receptor and is vastly expressed in immune cells and gastrointestinal cells, suggesting the potential physiological importance of GPR35 in these cells. Here, we tested the hypothesis that the lack of GPR35 expression in the colon mucosa exacerbates the severity of dextran sulfate sodium (DSS)-induced experimental colitis in mice.

Methods

Colitis was induced in GPR35 wild-type (GPR35^+/+) and GPR35 knockout (GPR35^-/-) mice through the administration of DSS in drinking water for 5 days followed by regular facility water for 1 day. Induction of colitis was evaluated by measuring relative body weight loss, clinical illness scores, and morphological changes in the colon. Abolition of Gpr35 gene exp

SUBMITTER: Farooq SM 

PROVIDER: S-EPMC6373462 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature