Nuclear position relative to the Golgi body and nuclear orientation are differentially responsive indicators of cell polarized motility.

ABSTRACT: Cell motility is critical to biological processes from wound healing to cancer metastasis to embryonic development. The involvement of organelles in cell motility is well established, but the role of organelle positional reorganization in cell motility remains poorly understood. Here we present an automated image analysis technique for tracking the shape and motion of Golgi bodies and cell nuclei. We quantify the relationship between nuclear orientation and the orientation of the Golgi body relative to the nucleus before, during, and after exposure of mouse fibroblasts to a controlled change in cell substrate topography, from flat to wrinkles, designed to trigger polarized motility. We find that the cells alter their mean nuclei orientation, in terms of the nuclear major axis, to increasin

SUBMITTER: Brasch ME 

PROVIDER: S-EPMC6373915 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature