Secretory Proteome of Brown Adipocytes in Response to cAMP-Mediated Thermogenic Activation.

ABSTRACT: Background: The secretory properties of brown adipose tissue are thought to contribute to the association between active brown fat and a healthy metabolic status. Although a few brown adipokines have been identified, a comprehensive knowledge of the brown adipose tissue secretome is lacking. Methods: Here, to examine the effects of thermogenic activation of brown adipocytes on protein secretion, we used isobaric tags for relative and absolute quantification (iTRAQ) analysis to determine how the secreted proteome of brown adipocytes (that detected in cell culture medium) differed in response to cAMP. Results: Our results indicated that 56 secreted proteins were up-regulated in response to cAMP. Of them, nearly half (29) corresponded to extracellular matrix components an

SUBMITTER: Villarroya J 

PROVIDER: S-EPMC6374321 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature