Dysregulation of autophagy in the central nervous system of sheep naturally infected with classical scrapie.

ABSTRACT: Autophagy is a dynamic cellular mechanism involved in protein and organelle turnover through lysosomal degradation. Autophagy regulation modulates the pathologies associated with many neurodegenerative diseases. Using sheep naturally infected with scrapie as a natural animal model of prion diseases, we investigated the regulation of autophagy in the central nervous system (CNS) during the clinical phase of the disease. We present a gene expression and protein distribution analysis of different autophagy-related markers and investigate their relationship with prion-associated lesions in several areas of the CNS. Gene expression of autophagy markers ATG5 and ATG9 was downregulated in some areas of scrapie brains. In contrast, ATG5 protein accumulates in medulla oblongata and positively corre

SUBMITTER: Lopez-Perez O 

PROVIDER: S-EPMC6374525 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature