DNA methylation profiling is a method of choice for molecular verification of pediatric WNT-activated medulloblastomas.

ABSTRACT:

Background

Wingless-activated medulloblastoma (WNT MB) represents a well-characterized molecular variant accounting for 10-15% of all MB and is associated with a favorable clinical outcome. Patients with localized WNT MBs could benefit from de-intensification of combined treatment, which would require an accurate diagnosis of these tumors. However, despite the presence of molecular features related with a WNT MB signature (nuclear ß-catenin immunoexpression, CTNNB1 mutation, and monosomy 6), a prompt and reliable diagnostic verification of these tumors is not yet feasible.

Methods

In the current study, we analyzed 78 samples of WNT MB treated in a single institute through genome-wide DNA methylation and targeted next generation sequencing to elaborate an optimal method for W

SUBMITTER: Korshunov A 

PROVIDER: S-EPMC6374763 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature