Identification and characterization of genes with absolute mRNA abundances changes in tumor cells with varied transcriptome sizes.

ABSTRACT:

Background

The amount of RNA per cell, namely the transcriptome size, may vary under many biological conditions including tumor. If the transcriptome size of two cells is different, direct comparison of the expression measurements on the same amount of total RNA for two samples can only identify genes with changes in the relative mRNA abundances, i.e., cellular mRNA concentration, rather than genes with changes in the absolute mRNA abundances.

Results

Our recently proposed RankCompV2 algorithm identify differentially expressed genes (DEGs) through comparing the relative expression orderings (REOs) of disease samples with that of normal samples. We reasoned that both the mRNA concentration and the absolute abundances of these DEGs must have changes in disease samples. In simu

SUBMITTER: Cai H 

PROVIDER: S-EPMC6374894 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature