Correlating Live Cell Viability with Membrane Permeability Disruption Induced by Trivalent Chromium.

ABSTRACT: Cr(III) is often regarded as a trace essential micronutrient that can be found in many dietary supplements due to its participation in blood glucose regulation. However, increased levels of exposure have been linked to adverse health effects in living organisms. Herein, scanning electrochemical microscopy (SECM) was used to detect variation in membrane permeability of single cells (T24) resulting from exposure to a trivalent Cr-salt, CrCl₃. By employing electrochemical mediators, ferrocenemethanol (FcMeOH) and ferrocenecarboxylic acid (FcCOO^-), initially semipermeable and impermeable, respectively, complementary information was obtained. Three-dimensional COMSOL finite element analysis simulations were successfully used to quantify the permeability coefficients of eac

SUBMITTER: Filice FP 

PROVIDER: S-EPMC6374964 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature