Aligned fibers enhance nerve guide conduits when bridging peripheral nerve defects focused on early repair stage.

ABSTRACT: Nerve conduits enhance nerve regeneration in the repair of long-distance peripheral nerve defects. To help optimize the effectiveness of nerve conduits for nerve repair, we developed a multi-step electrospinning process for constructing nerve guide conduits with aligned nanofibers. The alignment of the nerve guide conduits was characterized by scanning electron microscopy and fast Fourier transform. The mechanical performance of the nerve guide conduits was assessed by testing for tensile strength and compression resistance. The biological performance of the aligned fibers was examined using Schwann cells, PC12 cells and dorsal root ganglia in vitro. Immunohistochemistry was performed for the Schwann cell marker S100 and for the neurofilament protein NF200 in PC12 cells and dorsal root gan

SUBMITTER: Quan Q 

PROVIDER: S-EPMC6375037 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature