Broad spectrum detection of DNA damage by Repair Assisted Damage Detection (RADD).

ABSTRACT: Environmental exposures, reactive by-products of cellular metabolism, and spontaneous deamination events result in a spectrum of DNA adducts that if un-repaired threaten genomic integrity by inducing mutations, increasing instability, and contributing to the initiation and progression of cancer. Assessment of DNA adducts in cells and tissues is critical for genotoxic and carcinogenic evaluation of chemical exposure and may provide insight into the etiology of cancer. Numerous methods to characterize the formation of DNA adducts and their retention for risk assessment have been developed. However, there are still significant drawbacks to the implementation and wide-spread use of these methods, because they often require a substantial amount of biological sample, highly specialized expertise

SUBMITTER: Holton NW 

PROVIDER: S-EPMC6375075 | biostudies-literature | 2018 Jun - Jul

REPOSITORIES: biostudies-literature