Multicolour single-molecule tracking of mRNA interactions with RNP granules.

ABSTRACT: Ribonucleoprotein (RNP) granules are non-membrane-bound organelles that have critical roles in the stress response^1,2, maternal messenger RNA storage³, synaptic plasticity⁴, tumour progression^5,6 and neurodegeneration^7-9. However, the dynamics of their mRNA components within and near the granule surface remain poorly characterized, particularly in the context and timing of mRNAs exiting translation. Herein, we used multicolour single-molecule tracking to quantify the precise timing and kinetics of single mRNAs as they exit translation and enter RNP granules during stress. We observed single mRNAs interacting with stress granules and P-bodies, with mRNAs moving bidirectionally between them. Although translating mRNAs only interact with

SUBMITTER: Moon SL 

PROVIDER: S-EPMC6375083 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature