PmiRDiscVali: an integrated pipeline for plant microRNA discovery and validation.

ABSTRACT:

Background

MicroRNAs (miRNAs) constitute a well-known small RNA (sRNA) species with important regulatory roles. To date, several bioinformatics tools have been developed for large-scale prediction of miRNAs based on high-throughput sequencing data. However, some of these tools become invalid without reference genomes, while some tools cannot supply user-friendly outputs. Besides, most of the current tools focus on the importance of secondary structures and sRNA expression patterns for miRNA prediction, while they do not pay attention to miRNA processing for reliability check.

Results

Here, we reported a pipeline PmiRDiscVali for plant miRNA discovery and partial validation. This pipeline integrated the popular tool miRDeep-P for plant miRNA prediction, making PmiRDiscVali co

SUBMITTER: Yu D 

PROVIDER: S-EPMC6375137 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature