Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome.

ABSTRACT:

Background

Studies of the cystic fibrosis (CF) lung microbiome have consistently shown that lung function decline is associated with decreased microbial diversity due to the dominance of opportunistic pathogens. However, how this phenomenon is reflected in the metabolites and chemical environment of lung secretions remains poorly understood.

Methods

Here we investigated the microbial and molecular composition of CF sputum samples using 16S rRNA gene amplicon sequencing and untargeted tandem mass spectrometry to determine their interrelationships and associations with clinical measures of disease severity.

Results

The CF metabolome was found to exist in two states: one from patients with more severe disease that had higher molecular diversity and more Pseudomonas aerug

SUBMITTER: Quinn RA 

PROVIDER: S-EPMC6375204 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature