Engineering of CHO cells for the production of vertebrate recombinant sialyltransferases.

ABSTRACT:

Background

Sialyltransferases (SIATs) are a family of enzymes that transfer sialic acid (Sia) to glycan chains on glycoproteins, glycolipids, and oligosaccharides. They play key roles in determining cell-cell and cell-matrix interactions and are important in neuronal development, immune regulation, protein stability and clearance. Most fully characterized SIATs are of mammalian origin and these have been used for in vitro and in vivo modification of glycans. Additional versatility could be achieved by the use of animal SIATs from other species that live in much more variable environments. Our aim was to generate a panel of stable CHO cell lines expressing a range of vertebrate SIATs with different physicochemical and functional properties.

Methods

The soluble forms of variou

SUBMITTER: Houeix B 

PROVIDER: S-EPMC6375257 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature