A multi-amplicon 16S rRNA sequencing and analysis method for improved taxonomic profiling of bacterial communities.

ABSTRACT: Metagenomic sequencing of bacterial samples has become the gold standard for profiling microbial populations, but 16S rRNA profiling remains widely used due to advantages in sample throughput, cost, and sensitivity even though the approach is hampered by primer bias and lack of specificity. We hypothesized that a hybrid approach, that combined targeted PCR amplification with high-throughput sequencing of multiple regions of the genome, would capture many of the advantages of both approaches. We developed a method that identifies and quantifies members of bacterial communities through simultaneous analysis of multiple variable regions of the bacterial 16S rRNA gene. The method combines high-throughput microfluidics for PCR amplification, short read DNA sequencing, and a custom algorithm nam

SUBMITTER: Schriefer AE 

PROVIDER: S-EPMC6375304 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature