Identification of molecular biomarkers for the diagnosis of gastric cancer and lymph-node metastasis.

ABSTRACT:

Background and objective

Biomarkers are important tools for prompt diagnosis of cancer. This study aimed to identify reliable biomarkers for clinical applications in the diagnosis of gastric cancer and lymph-node (LN) metastasis.

Methods

Between 1 December 2014 and 31 December 2015, we prospectively collected samples of gastric-cancer tissues, corresponding matched-pair normal gastric mucosa, and their peri-gastric metastatic and non-metastatic LNs to identify quantitatively reliable genes using quantitative real-time polymerase chain reaction. Relative quantity (RQ) was used to calculate the mRNA expression levels of our target genes. Statistics were calculated using one-way analysis of variance (ANOVA) and Tukey's multiple comparison test. Analytical graphs were plotted us

SUBMITTER: Seeruttun SR 

PROVIDER: S-EPMC6375354 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature