A rapid methods development workflow for high-throughput quantitative proteomic applications.

ABSTRACT: Recent improvements in the speed and sensitivity of liquid chromatography-mass spectrometry systems have driven significant progress toward system-wide characterization of the proteome of many species. These efforts create large proteomic datasets that provide insight into biological processes and identify diagnostic proteins whose abundance changes significantly under different experimental conditions. Yet, these system-wide experiments are typically the starting point for hypothesis-driven, follow-up experiments to elucidate the extent of the phenomenon or the utility of the diagnostic marker, wherein many samples must be analyzed. Transitioning from a few discovery experiments to quantitative analyses on hundreds of samples requires significant resources both to develop sensitive and sp

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6375547 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature