Assessing effects of germline exposure to environmental toxicants by high-throughput screening in C. elegans.

ABSTRACT: Chemicals that are highly prevalent in our environment, such as phthalates and pesticides, have been linked to problems associated with reproductive health. However, rapid assessment of their impact on reproductive health and understanding how they cause such deleterious effects, remain challenging due to their fast-growing numbers and the limitations of various current toxicity assessment model systems. Here, we performed a high-throughput screen in C. elegans to identify chemicals inducing aneuploidy as a result of impaired germline function. We screened 46 chemicals that are widely present in our environment, but for which effects in the germline remain poorly understood. These included pesticides, phthalates, and chemicals used in hydraulic fracturing and crude oil processing. Of the 4

SUBMITTER: Shin N 

PROVIDER: S-EPMC6375566 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature