Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.

ABSTRACT: Mechanobiology, the study of how mechanical forces affect cellular behavior, is an emerging field of study that has garnered broad and significant interest. Researchers are currently seeking to better understand how mechanical signals are transmitted, detected, and integrated at a subcellular level. One tool for addressing these questions is a Förster resonance energy transfer (FRET)-based tension sensor, which enables the measurement of molecular-scale forces across proteins based on changes in emitted light. However, the reliability and reproducibility of measurements made with these sensors has not been thoroughly examined. To address these concerns, we developed numerical methods that improve the accuracy of measurements made using sensitized emission-based imaging. To establish that F

SUBMITTER: Gates EM 

PROVIDER: S-EPMC6375766 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature