C5aR1 regulates migration of suppressive myeloid cells required for costimulatory blockade-induced murine allograft survival.

ABSTRACT: Costimulatory blockade-induced murine cardiac allograft survival requires intragraft accumulation of CD11b⁺ Ly6C^lo Ly6G^- regulatory myeloid cells (Mregs) that expand regulatory T cells (Tregs) and suppress effector T cells (Teffs). We previously showed that C5a receptor (C5aR1) signaling on T cells activates Teffs and inhibits Tregs, but whether and/or how C5aR1 affects Mregs required for transplant survival is unknown. Although BALB/c hearts survived >60 days in anti-CD154 (MR1)-treated or cytotoxic T-lymphocyte associated protein 4 (CTLA4)-Ig-treated wild-type (WT) recipients, they were rejected at ~30 days in MR1-treated or CTLA4-Ig-treated recipients selectively deficient in C5aR1 restricted to myeloid cells (C5ar1^fl/fl xLysM-Cre). This accel

SUBMITTER: Llaudo I 

PROVIDER: S-EPMC6375810 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature