Annexin II Light Chain p11 Interacts With ENaC to Increase Functional Activity at the Membrane.

ABSTRACT: The epithelial Na⁺ channel (ENaC) provides for Na⁺ absorption in various types of epithelia including the kidney, lung, and colon where ENaC is localized to the apical membrane to enable Na⁺ entry into the cell. The degree of Na⁺ entry via ENaC largely depends on the number of active channels localized to the cell membrane, and is tightly controlled by interactions with ubiquitin ligases, kinases, and G-proteins. While regulation of ENaC endocytosis has been well-studied, relatively little is understood of the proteins that govern ENaC exocytosis. We hypothesized that the annexin II light chain, p11, could participate in the transport of ENaC along the exocytic pathway. Our results demonstrate that all three ENaC channel subunits interacted with

SUBMITTER: Cheung TT 

PROVIDER: S-EPMC6375906 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature