The long non-coding RNA Meg3 is dispensable for hematopoietic stem cells.

ABSTRACT: The long non-coding RNA (lncRNA) Maternally Expressed Gene 3 (Meg3) is encoded within the imprinted Dlk1-Meg3 gene locus and is only maternally expressed. Meg3 has been shown to play an important role in the regulation of cellular proliferation and functions as a tumor suppressor in numerous tissues. Meg3 is highly expressed in mouse adult hematopoietic stem cells (HSCs) and strongly down-regulated in early progenitors. To address its functional role in HSCs, we used MxCre to conditionally delete Meg3 in the adult bone marrow of Meg3^{mat-flox/pat-wt} mice. We performed extensive in vitro and in vivo analyses of mice carrying a Meg3 deficient blood system, but neither observed impaired hematopoiesis during homeostatic conditions nor upon serial transplantation. Furthermore, we anal

SUBMITTER: Sommerkamp P 

PROVIDER: S-EPMC6375991 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature