Autofluorescence is a Reliable in vitro Marker of Cellular Senescence in Human Mesenchymal Stromal Cells.

ABSTRACT: Mesenchymal stromal cells (MSC) are used in cell therapies, however cellular senescence increases heterogeneity of cell populations and leads to uncertainty in therapies' outcomes. The determination of cellular senescence is time consuming and logistically intensive. Here, we propose the use of endogenous autofluorescence as real-time quantification of cellular senescence in human MSC, based on label-free flow cytometry analysis. We correlated cell autofluorescence to senescence using senescence-associated beta-galactosidase assay (SA-β-Gal) with chromogenic (X-GAL) and fluorescent (C₁₂FDG) substrates, gene expression of senescence markers (such as p16^INK4A, p18^INK4C, CCND2 and CDCA7) and telomere length. Autofluorescence was further correlated to MSC diffe

SUBMITTER: Bertolo A 

PROVIDER: S-EPMC6376004 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature