A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for flubendiamide detection.

ABSTRACT: Flubendiamide (FD), the first commercial phthalic acid diamide that targets insect ryanodine receptor (RyRs), has played an important role in pest management. With its extensive worldwide application, a rapid and convenient method to detect its existence in the environment is necessary. In this study, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to analyse FD residue on environmental and food samples. The established icELISA showed a half maximal inhibition concentration (IC₅₀) of 17.25 µg L^-1, with a working range of 4.06-103.59 µg L^-1 for FD, and showed no cross-reactivity with chlorantraniliprole, cyantraniliprole, and several FD analogues. Average FD recoveries from spinach, tap water, and soil samples were 89.3-112.

SUBMITTER: Li Q 

PROVIDER: S-EPMC6376021 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature