High sensitivity detection of extracellular vesicles immune-captured from urine by conventional flow cytometry.

ABSTRACT: Extracellular vesicles (EVs) provide an invaluable tool to analyse physiological processes because they transport, in biological fluids, biomolecules secreted from diverse tissues of an individual. EV biomarker detection requires highly sensitive techniques able to identify individual molecules. However, the lack of widespread, affordable methodologies for high-throughput EV analyses means that studies on biomarkers have not been done in large patient cohorts. To develop tools for EV analysis in biological samples, we evaluated here the critical parameters to optimise an assay based on immunocapture of EVs followed by flow cytometry. We describe a straightforward method for EV detection using general EV markers like the tetraspanins CD9, CD63 and CD81, that allowed highly sensitive detecti

SUBMITTER: Campos-Silva C 

PROVIDER: S-EPMC6376115 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature