RNA-seq analysis of PHD and VHL inhibitors reveals differences and similarities to the hypoxia response.

ABSTRACT: Background: Hypoxia-inducible factor (HIF) transcription factors are well known to control the transcriptional response to hypoxia. Given the importance of cellular response to hypoxia, a number of pharmacological agents to interfere with this pathway have been developed and entered pre-clinical or clinical trial phases. However, how similar or divergent the transcriptional response elicited by different points of interference in cells is currently unknown. Methods: We performed RNA-sequencing to analyse the similarities and differences of transcriptional response in HeLa cells treated with hypoxia or chemical agents that stabilise HIF by inhibiting components of the hypoxia signalling pathway - prolyl hydroxylase (PHD) inhibitor or von Hippel-Lindau (VHL) inhibitor. Resul

SUBMITTER: Frost J 

PROVIDER: S-EPMC6376255 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature