Ezrin, a Membrane Cytoskeleton Cross-Linker Protein, as a Marker of Epithelial Damage in Asthma.

ABSTRACT:

Rationale

Bronchial epithelial cell damage occurs in patients with bronchial asthma. Ezrin, a membrane-cytoskeleton protein, maintains cellular morphology and intercellular adhesion and protects the barrier function of epithelial cells.

Objectives

To study the role of ezrin in bronchial epithelial cells injury and correlate its expression with asthma severity.

Methods

Levels of ezrin were measured in exhaled breath condensate (EBC) and serum in patients with asthma and BAL fluid (BALF) from a mouse model of asthma by ELISA. The regulation of IL-13 on ezrin protein levels was studied in primary bronchial epithelial cells. Ezrin knockdown using shRNA was studied in human bronchial epithelial 16HBE cells.

Measurements and main results

Ezrin levels were decreased i

SUBMITTER: Jia M 

PROVIDER: S-EPMC6376623 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature