Double bonds of unsaturated fatty acids differentially regulate mitochondrial cardiolipin remodeling.

ABSTRACT:

Background

Supplemented fatty acids can incorporate into cardiolipin (CL) and affect its remodeling. The change in CL species may alter the mitochondrial membrane composition, potentially disturbing the mitochondrial structure and function during inflammation.

Method

To investigate the effect of the unsaturation of fatty acids on CL, we supplemented macrophage-like RAW264.7 cells with 18-carbon unsaturated fatty acids including oleic acid (OA, 18:1), linoleic acid (LA, 18:2), α-linolenic acid (ALA, 18:3), γ-linolenic acid (GLA, 18:3), and stearidonic acid (SDA, 18:4). Mitochondrial changes in CL were measured through mass spectrometry.

Result

Our data indicated that OA(18:1) was the most efficient fatty acid that incorporated into CL, forming symmetrical CL without fa

SUBMITTER: Ting HC 

PROVIDER: S-EPMC6376731 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature