Activated Wnt signaling promotes growth and progression of AFP-producing gastric cancer in preclinical models.

ABSTRACT:

Background

Characterized by elevated AFP levels in serum, AFP-producing gastric cancer (APGC) is a very special type of gastric cancer (GC) that is difficult to treat and has poor prognosis. However, little is known about the role of AFP in GC, which was investigated in this study with in vitro and in vivo experiments.

Methods

APGC cells were established with lentivirus infection and validated by PCR assay and ELISA in HCG27 and AGS cells. Cell growth, migration, and invasion were determined by CCK8, transwell assays, and animal experiments. RNA sequencing, Western blot, dual-luciferase-reporter assays, and RNA interference were employed to understand mechanisms underlying AFP activity, followed by therapeutic investigations for APGC.

Results

APGC cells featured signi

SUBMITTER: Chen D 

PROVIDER: S-EPMC6376882 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature