Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues.

ABSTRACT: Cell-laden microgels have been used as tissue building blocks to create three-dimensional (3D) tissues and organs. However, traditional assembly methods can not be used to fabricate functional tissue constructs with biomechanical and structural complexity. In this study, we present directed assembly of cell-laden dual-crosslinkable alginate microgels comprised of oxidized and methacrylated alginate (OMA). Cell-laden OMA microgels can be directly assembled into well-defined 3D shapes and structures under low-level ultraviolet light. Stem cell-laden OMA microgels can be successfully cryopreserved for long-term storage and on-demand applications, and the recovered encapsulated cells maintained equivalent viability and functionality to the freshly processed stem cells. Finally, we have success

SUBMITTER: Jeon O 

PROVIDER: S-EPMC6377241 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature