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The Majority of CD45- Ter119- CD31- Bone Marrow Cell Fraction Is of Hematopoietic Origin and Contains Erythroid and Lymphoid Progenitors.

ABSTRACT: The non-hematopoietic cell fraction of the bone marrow (BM) is classically identified as CD45- Ter119- CD31- (herein referred to as triple-negative cells or TNCs). Although TNCs are believed to contain heterogeneous stromal cell populations, they remain poorly defined. Here we showed that the vast majority of TNCs (?85%) have a hematopoietic rather than mesenchymal origin. Single cell RNA-sequencing revealed erythroid and lymphoid progenitor signatures among CD51- TNCs. Ly6D+ CD44+ CD51- TNCs phenotypically and functionally resembled CD45+ pro-B lymphoid cells, whereas Ly6D- CD44+ CD51- TNCs were enriched in previously unappreciated stromal-dependent erythroid progenitors hierarchically situated between preCFU-E and proerythroblasts. Upon adoptive transfer, CD44+ CD51- TNCs contributed to repopulate the B-lymphoid and erythroid compartments. CD44+ CD51- TNCs also expanded during phenylhydrazine-induced acute hemolysis or in a model of sickle cell anemia. These findings thus uncover physiologically relevant new classes of stromal-associated functional CD45- hematopoietic progenitors.

SUBMITTER: Boulais PE 

PROVIDER: S-EPMC6377266 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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The Majority of CD45<sup>-</sup> Ter119<sup>-</sup> CD31<sup>-</sup> Bone Marrow Cell Fraction Is of Hematopoietic Origin and Contains Erythroid and Lymphoid Progenitors.

Boulais Philip E PE   Mizoguchi Toshihide T   Zimmerman Samuel S   Nakahara Fumio F   Vivié Judith J   Mar Jessica C JC   van Oudenaarden Alexander A   Frenette Paul S PS  

Immunity 20181009 4

The non-hematopoietic cell fraction of the bone marrow (BM) is classically identified as CD45<sup>-</sup> Ter119<sup>-</sup> CD31<sup>-</sup> (herein referred to as triple-negative cells or TNCs). Although TNCs are believed to contain heterogeneous stromal cell populations, they remain poorly defined. Here we showed that the vast majority of TNCs (∼85%) have a hematopoietic rather than mesenchymal origin. Single cell RNA-sequencing revealed erythroid and lymphoid progenitor signatures among CD51  ...[more]

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