Quantification of reaction cycle parameters for an essential molecular switch in an auxin-responsive transcription circuit in rice.

ABSTRACT: Protein-based molecular switches play critical roles in biological processes. The importance of the prolyl cis-trans switch is underscored by the ubiquitous presence of peptidyl prolyl isomerases such as cyclophilins that accelerate the intrinsically slow isomerization rate. In rice, a tryptophan-proline (W-P) cis-trans switch in transcription repressor protein OsIAA11 along with its associated cyclophilin LRT2 are essential components in a negative feedback gene regulation circuit that controls lateral root initiation in response to the plant hormone auxin. Importantly, no quantitative characterizations of the individual (microscopic) thermodynamic and kinetic parameters for any cyclophilin-catalyzed W-P isomerization have been reported. Here we present NMR stu

SUBMITTER: Acevedo LA 

PROVIDER: S-EPMC6377490 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature