Reliable reference genes for expression analysis of proliferating and adipogenically differentiating human adipose stromal cells.

ABSTRACT:

Background

The proliferation and adipogenic differentiation of adipose stromal cells (ASCs) are complex processes comprising major phenotypical alterations driven by up- and downregulation of hundreds of genes. Quantitative RT-PCR can be employed to measure relative changes in the expression of a gene of interest. This approach requires constitutively expressed reference genes for normalization to counteract inter-sample variations due to differences in RNA quality and quantity. Thus, a careful validation of quantitative RT-PCR reference genes is needed to accurately measure fluctuations in the expression of genes. Here, we evaluated candidate reference genes applicable for quantitative RT-PCR analysis of gene expression during proliferation and adipogenesis of human ASCs with the

SUBMITTER: Krautgasser C 

PROVIDER: S-EPMC6377720 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature