Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens.

ABSTRACT:

Background

Despite a number of different transgenes that can mediate DNA deletion in the developing lens, each has unique features that can make a given transgenic line more or less appropriate for particular studies. The purpose of this work encompasses both a review of transgenes that lead to the expression of Cre recombinase in the lens and a comparative analysis of currently available transgenic lines with a particular emphasis on the Le-Cre and P0-3.9GFPCre lines that can mediate DNA deletion in the lens placode. Although both of these transgenes are driven by elements of the Pax6 P0 promoter, the Le-Cre transgene consistently leads to ocular abnormalities in homozygous state and can lead to ocular defects on some genetic backgrounds when hemizygous.

Result

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SUBMITTER: Lam PT 

PROVIDER: S-EPMC6377743 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature